Regarding the Q-LED indicator, please refer to the following link to troubleshoot the problem you might be having.
Please help provide the complete model of the memory or go to the support page of TUF GAMING B650-PLUS
to confirm whether the memory you purchased is listed in the QVL list. It is recommended that you use the memory in the QVL list to ensure compatibility.
Also, please help with the following attempts:
1. Default BIOS setting (clean CMOS) to confirm whether you can boot into the system normally
2. When installing one memory module, insert it into the DIMM_A2 slot; when installing two memory modules, insert them into the DIMM_A2 and DIMM_B2 slots.
Please test whether the system can boot normally when installing one memory module and then repeat the test with two memory modules installed.
For more information about AI Overclock Tuner, please refer to page 17 of the BIOS Manual.