Some additional things to try/troubleshoot that I always look at if I see issues after updating drivers, etc. I don't know why any specific item would interact with ThunderboltEx4
*) Remove any memory overclocks for a moment.
*) Make sure you're set to Windows Secure Boot in the Bios
*) I used Single Root Virtualization
*) Turn off Fast Boot
*) In the Thunderbolt section if you're using WIndows 11, turn OFF the win10 support
*) Enable the GNA module
*) I turned on PowerManagement and Hot Plug for the media controller.
Save & Exit, then shutdown to turn the machine FULLY off.
Turn the machine fully off, Plug in the TB devices, then power up from an OFF state.
In my experience, yes, Discrete Thunderbolt(TM) Support needs to be on.
I wish I knew exactly what setting caused it to start working. I went through turning ON/UP all of the Windows security options as well as changing the BIOS settings above.
Here's an image of what the control center looks like when working.
Note that I didn't have to do anything inside of the control center software itself, it was some combination of windows settings + BIOS settings + PCIE Slot change that unlocked PCIE tunneling.
Could you please help to check if the Thunderboltex 4 card in other PCIE slot, does it also show disabled?
like exchange PCIEX16_2 and PCIEX16_3 or only install graphics card and Thunderboltex 4 card in PCIEX16_2 or PCIEX16_3