Depends on the slots you use. The Maximus IV has a NF200 chip on it, so technically you could get x16 and x16, but at the cost of lag from the additional NF200 chip, although I doubt you could tell. Keeping that in mind, if you look in your manual it actually suggests which slots to use depending on how many cards you are using. If you use the first slot and any other slot, the first card would be at x8 since it is working off the Intel chipset and the second card would be at either x8 (working off the Intel chipset) or x16 using the NF200 ship. Regardless of how you are going to set it up, the difference between x8 and x16 is maybe 2-3 FPS. I would use the the cards in the slots controlled by the Intel chipset and run them at x8 and x8.