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Strix Z490-G M.2 slot 1 stopped working after PSU upgrade!?

Level 7
Yes, you read right! Need help!

I bough a new PSU for upgrade, nothing wrong with my old Ax860i.

Put the new PSU in and fired the computer up. came to bios. No drives showing up as boot option.

Have 2 Intel 660P m.2 in slot 1 and 2. (256gb and 1tb) and i storage 2.5" HDD (tb) in Sata 6 slot 1. (having these like this like 6months)

Bios finds the second M.2 and HDD. but cant figure out why the first slot is not showing?
The bios was resetted when i started the computer after change.

If i move the M.2 with windows on to slot 2 its shows up and can boot to windows..
Tried remove the GPU also if that was the issue with pci lanes, but no..

The slot cant just go bad like that? feels more like its disabled?

Im not a pro and hope i can get some help..

If i cant get the slot 1 working again, can i use slot 2 only without performace issue?