ASUS B650E-E Nvme M2 SSD configuration

[Orodruin]

ASUS B650E-E Nvme M2 SSD support

Hello everyone. I am using ASUS B650E-E motherboard. There are a total of 4 M2 slots on this motherboard.
And I use them all.
This motherboard is structured as follows.

M2_1 PCIe 5.0 (Cpu Line)
M2_2 PCIe 4.0 (Cpu Line)
M2_3 Pcie 5.0 (Cpu Line) (If M2 is inserted into this slot, the GPU PCIE X16 bus drops to X8.)
M2_4 Pice 4.0 (This slot comes from the chipset)

Apart from these, there are 3 PCIE slots.
PCIEX16_1 (X16 Speed ​​Gen 5.0)
PCIEX16_2 (X4 Speed ​​Gen 5.0)
PCIEX16_3 (X4 Speed ​​Gen 4.0)

Now my question is;
While 4 M2 slots are occupied, will there be a problem if I add one more M2 to the last PCIEX16_3 slot? Does it affect my work speed?

Will I have a problem with PCIE Lines?

https://www.asus.com/support/faq/1037507/
When I look at the Hyper M2 configuration in this link, I see that it can work.
If anyone has any knowledge and advice, I would be very happy.